To determine if Prdm16 haploinsufficiency contributes to hearing deficits in mice and to understand the pathophysiology of hearing loss secondary to Prdm16 haploinsufficiency in 1p36 deletion syndrome (Heilstedt et al., 2003; Battaglia et al., 2008; Shimada et al., 2015; Jacquin et al., 2023), we compared Prdm16 heterozygote hearing to littermate wild-type mice. This evidence concerns the gene PRDM16 and Down syndrome.