Our gene expression profiling and multiplex spatial imaging of tumor specimens, along with the analysis of spatial relationships among CAFs and immune cells, revealed a significant increase in the proportion of immune-excluded tumors among mTLS-positive NSCLC with high-density levels of FAP+αSMA+ CAFs and MYH11+SMA+ CAFs. The gene discussed is MYH11; the disease is non-small cell lung carcinoma.