In 3 out of 6 patients with AML harboring pathogenic or likely pathogenic CSF3R variants, a variant allele frequency (VAF) of 44.0%, 41.8%, 38.9% and 38.6% characterized these mutations, suggesting a dominant distribution inside the leukemic population, whereas lower VAF were detected in the other 3 cases compared to other co-occurring mutations, implying a subclonal pattern in these cases. This evidence concerns the gene CSF3R and acute myeloid leukemia.