These tracers were (i) confirmed as high affinity, non-discriminating TSPO binders in HEK cells transfected with WT or A147T TSPO and human brain tissue from genotyped multiple sclerosis patients, (ii) characterised for biodistribution and metabolic stability in WT rats, and (iii) evaluated for the ability to image neuroinflammation in an EAE rat model of multiple sclerosis. The gene discussed is TSPO; the disease is multiple sclerosis.