NVL and systemic sclerosis: Moreover, Perurena-Prieto et al. reported the detection of anti-NVL AAb and homogenous nucleolar HEp-2 cell staining (AC-8) for six out of 307 SSc patients (2.0, 95% CI: 0.7–4.2%) using non-radioactive immunoprecipitation, Western blot assay, and liquid mass spectrometry (16).