To determine the effect of DP71L on antiviral signaling cascades, we examined phosphorylation of key molecules involved in the signaling pathway, including TBK1, STAT1, IRF3, p65 (RelA), and NF-κB inhibitor alpha (IκBα), in DP71L-transfected PK-15 cells and DP71L stably expressing 3D4/21 cells following infection with ADV-GFP. Here, IRF3 is linked to infection.