In the lower chamber, we grew five different cancer cell lines: breast cancer cells MCF-7 (estrogen receptor-positive (ER+), progesterone receptor-positive (PR+), glucocorticoid receptor-positive (GR+)) and MDA-MB-436 (triple negative), colon cancer (SW-1417), pancreatic cancer (Panc 10.05) and prostate cancer (DU-145). Here, ESR1 is linked to breast cancer.