Using Real-time PCR (Fig. 5J), flow cytometry (Fig. 5K, Supplementary Fig. 4I), and IF assays (Fig. 5L), we found that the expression level of CD80 decreased, whereas the levels of CD163, CD206, and IL-10 increased in THP-1 cells after incubation with the CM of the GP73 overexpression group of HCC cells, and PKM2 knockdown by shRNA partially reversed these effects. This evidence concerns the gene CD163 and hepatocellular carcinoma.