To further investigate the role of GP73-mediated PKM2 and GP73 secretion in the TME of HCC, HCC cells with stable expression of Vector, GP73, or GP73+shPKM2 were constructed and co-cultured with PMA-treated THP-1 cells for 24 h, and the conditioned medium (CM) was collected. The gene discussed is PKM; the disease is hepatocellular carcinoma.