With deletion of Pten there is evidence of metabolic reprogramming in prostate cancer cells [27].To understand the anti-cancer potential of I3C, particularly at metabolomic level in prostate cancer based Pten KO mice model, we treated mice non-invasively with AIN-93 M diet containing 1% (w/w) I3C for 20 weeks. The gene discussed is PTEN; the disease is cancer.