In addition, the upregulation of Lgals3 was further confirmed in the kidneys of nephrolithiasis patients by western blot and qPCR (Figure 1J–K).To assess the clinical relevance of Lgals3 in CaOx stone, 10 kidney tissues from patients with CaOx kidney stone patients and 10 kidney tissues from radical nephrectomy for renal cell carcinoma were analyzed, showing a significant increase in Lgals3 expression in patients with kidney stone, which correlated closely with kidney function (Figure 1L–N). Here, LGALS3 is linked to hereditary clear cell renal cell carcinoma.