To detect whether cholesterol plays a role in the invasion and metastasis of HCC caused by RNASET2 deficiency, we treated HCC cells with methyl‐β‐cyclodextrin (MβCD) to deplete cholesterol and then supplemented them with exogenous cholesterol to induce cholesterol accumulation (Figure S5A, Supporting Information). Here, RNASET2 is linked to hepatocellular carcinoma.