To further validate the effect of SYNGR4 on macrophage polarization in the tumor immune microenvironment, we analyzed macrophages in the tumors of mice in the SYNGR4 knockdown and control groups by flow cytometry, and the results showed an increased polarization of macrophages toward M1 and a significant decrease in M2-like macrophages in breast cancers of mice with SYNGR4 knockdown relative to controls (Figure 7F). This evidence concerns the gene SYNGR4 and breast cancer.