We performed bulk RNA sequencing (RNA-seq) on Fluorescence-Activated Cell Sorted (FACS) CD4+ T cells from cryopreserved peripheral blood mononuclear cells (PBMC) of four men and four women acutely infected with HIV-1 [approximately 3 months post–estimated date of infection (EDI); median, 86.5 days post-EDI]. Here, CD4 is linked to infection.