The outcomes of RT-qPCR analysis showed that the expressions of MAP3K1, MAP3K4, MAP3K5, MAP3K6, MAP3K7, MAP3K8, MAP3K9, and MAP3K10 were significantly (p-value < 0.05) up-regulated in GC cell lines relative to control cell lines (Fig. 1A), while the expression of MAP3K2, MAP3K3, MAP3K11, MAP3K12, MAP3K13, MAP3K14, and MAP3K15 was significantly (p-value < 0.05) down-regulated in GC cell lines relative to control cell lines (Fig. 1B). The gene discussed is MAP3K4; the disease is gastric cancer.