Silencing GPX4 by RNA interference and exposure to tert-butyl hydroperoxide (tert-BHP) induced ferroptosis in rat pancreatic β-cells, while GPX4 overexpression and Fer-1 effectively attenuated β-cell death, confirming Inhibiting GPX4 activity may promote β-cell death in T1DM. This evidence concerns the gene GPX4 and type 1 diabetes mellitus.