Multicolor immunofluorescence demonstrated that cinobufagin enhanced the expression of cleaved N-terminal gasdermin-E (N-GSDME, a pyroptosis executor), cleaved caspase-3 (c-CASP3, an apoptosis executor), and phosphorylated mixed lineage kinase domain-like protein (p-MLKL, a necroptosis executor) in GBM tumor cells. The gene discussed is MLKL; the disease is glioblastoma.