Flow cytometry analysis revealed an increase in the proliferative (Ki67+), activated (CD69+), and cytotoxic (TNFα+, IFNγ+, GzmB+) CD8+ and CD4+ T cells in tumor tissues (Fig. 10A-D) and spleens (Supplement Fig. 12I-L) of GL261 tumor-bearing mice treated with cinobufagin, compared to the vehicle-treated group. This evidence concerns the gene GZMB and neoplasm.