Using SenTraGor (STG, a reagent for detection of lipofuscin) [50] staining in postmortem human brain slices (mid-frontal lobe region) from ROS, we observed higher STG intensity in GFAP-positive astrocytes in AD participants compared to NCI participants with the APOE3/4 genotype (Fig. 2A, the STG/GFAP staining test is shown in Supplementary Fig. 2A), corroborating the increased astrocyte senescence markers in AD observed in the transcriptomic data. Here, GFAP is linked to Alzheimer disease.