While there was also no difference in the proportion of MANAscorehi TIL from specific clusters in responding and non-responding tumors (Supplementary Fig. 11C), the pseudobulk gene expression profile of MANAscorehi TIL showed a marginally significant canonical correlation with percent residual tumor (canonical correlation: 0.59, p = 0.05) (Supplementary Fig. 11D), but not with discrete response (MPR vs non-MPR) to PD-1 blockade (canonical correlation: 0.48, p = 0.18) (Supplementary Fig. 11D). Here, PDCD1 is linked to neoplasm.