Analyzes of the above-described recent single cell studies in our NSCLC cohort and melanoma cohort directly identifying and functionally validating MANA-, EBV-, and Influenza (flu)-specific T cell clones at the TCR level8,9 provided a unique ground truth dataset from which to test and validate gene expression patterns potentially specific for MANA-specific CD8+ TIL. Here, CD8A is linked to melanoma.