The dysregulation of the Hedgehog (Hh)-Gli signaling pathway is increasingly recognized as a key factor in the development of various human cancers, including basal cell carcinoma (BCC) [54], medulloblastoma (MB) [55], and embryonal rhabdomyosarcoma (eRMS) [56, 57], which are the three primary tumors associated with Gorlin syndrome [58]. This evidence concerns the gene GLI1 and skin basal cell carcinoma.