Because peroxisomes are essential for the transport of cholesterol into the ciliary membrane (Miyamoto et al, 2020), we generated Pex14-knockout mIMCD3 cells as a Zellweger syndrome model using the nonhomologous end-joining (NHEJ)–mediated targeting method named ObLiGaRe (obligate ligation-gated recombination) (Maresca et al, 2013; Royba et al, 2017) (Tables S1 and S2) to evaluate whether ciliary cholesterol modulates the localization of the polycystin complex. The gene discussed is PEX14; the disease is Zellweger syndrome.