Notably, silence of VANGL2 or MARCH8 enhanced the expression of IL-1β, Casp1, and NLRP3 in healthy PBMCs, but could not further promote the expression of IL-1β, Casp1, and NLRP3 in IBD PBMCs under the condition of NLRP3 inflammasome activation (Fig 8J–8M). Here, MARCHF8 is linked to inflammatory bowel disease.