For instance, Hsieh et al. (2011) found that lunasin can synergize with anacardic acid to produce a stronger anti-breast cancer effect, manifesting in the fact that the combination of them induced stronger toxicity to breast cancer cells, inhibited the growth of breast cancer cells more efficiently, led to more cell cycle arrest and apoptosis, and more significantly regulated the expression of genes related to cell cycle and DNA damage repair, such as CCNE1, CDK2, CDK4, and E2F1. The gene discussed is E2F1; the disease is breast carcinoma.