Compared with the co-expression ratio of TBX3+ CD8+ T cells (0.02%) and TBX3+ α-SMA+ CAFs (1.02%), TBX3 was mainly expressed in BLCA cells (71.28%), which revealed similar results as the scRNA sequencing (Figures 7C-D). This evidence concerns the gene ACTA1 and bladder transitional cell carcinoma.