Further, the knockdown of TGFβ1 also significantly inhibited the formation of an immunosuppressive microenvironment by increasing the percentage of CD8+ T cells (Figure S6F), GZMB+ CD8+ T cells (Figure S6G), and decreasing CAFs (Figure S6H) in the TME of BLCA. The gene discussed is GZMB; the disease is bladder transitional cell carcinoma.