The nuclear PKM2 stimulates the function of the reverse transcriptional activation domain of HIF-1α, and binds the hypoxia response element of the HIF-1α target gene recruited by P300 to the HRE site on the PD-L1 promoter, thereby upregulating the expression of PD-L1 on TAM, DC, T cells, and CT26 colon cancer cells. The gene discussed is CD274; the disease is malignant colon neoplasm.