To determine the effect of USP5 on in vivo NPC cell radiosensitivity, we generated subcutaneous tumors in nude mice using 5-8F NPC cells with USP5 knockdown, 5-8F NPC cells with USP5 knockdown and EphA2 overexpression, and shNC control 5-8F cells respectively (Fig. S4B). The gene discussed is EPHA2; the disease is nasopharyngeal carcinoma.