To further evaluate whether the effect of TTC7B on the proliferation of colon cancer cells is dependent on the demethylase activity of FTO, we constructed a special wild-type FTO expression vector containing the FTO promoter (FTO-wt), which is responsible for the regulatory effect of the TTC7B-RXRA axis on FTO transcription. The gene discussed is MBD2; the disease is malignant colon neoplasm.