Additionally, prophylactic treatment with antibiotics prevents the amplification of AP via the intestinal‒pancreatic axis by restraining the activation of TLR4/NLRP3 signaling in the colon, downregulating the expression of proinflammatory factors, promoting the expression of intestinal TJ-related proteins, improving the morphology of the intestine, and reducing the translocation of intestinal bacteria 114. This evidence concerns the gene TLR4 and alkaline phosphatase measurement.