These transcriptional data are consistent with our cytokine profiling results where MIF protein expression was readily detected in both tissue lysates obtained from specimens with high tumor cellularity as well as from conditioned media from patient-derived osteosarcoma cell lines cultured in vitro whereas CXCL12 protein expression was below the detection threshold (Figure 3D-E). This evidence concerns the gene CXCL12 and osteosarcoma.