Among KC-specific TFs, Hand2 and Tbx1 are the major transcription factors that regulate vascularization (Cioffi et al., 2014; Dai and Cserjesi, 2002; Morikawa and Cserjesi, 2004; Vitelli et al., 2002), which is the main cause of corneal opacity. The gene discussed is CALCA; the disease is Corneal opacity.