Similarly to the last study using WT rats, presymptomatic ALS rats (75 days old) were unilaterally injected with their respective treatment (Bet1L siRNA, scrambled siRNA, vehicle only, or sham; n = 4 each group) into their TA muscles, and we analyzed NMJ innervation and Bet1L expression by immunohistochemistry (Figure 3C). Here, BET1L is linked to amyotrophic lateral sclerosis.