We analyzed the expression of the SOX2 regulatory network in a public scRNA-seq dataset of patient SHH-MB.20 Upon clustering tumor cells in Seurat13 a single cluster in 1 of the 3 SHH-MB tumors expressed a highly similar network in which OTX2 was replaced by expression of another member of the regulatory network, SRRT (Figures 3A and 4A).43 Furthermore, in 1 of the 2 PDX models of SHH-MB from the same dataset,20 a single cluster expressed a common network, consisting of POU3F2, OTX2, SRRT, GLI2, and SOX2 (Figure 4A). Here, GLI2 is linked to neoplasm.