In comparison, genes upregulated in tumor spheroids were associated with altered cell morphology and wound healing for example IFITM3, IGFBP2, IGFBP5, STC1, and SPARC (Supplementary Figure S2B—C and Table S3) consistent with an epithelial-to-mesenchymal transition state and tumor stroma production.25 Upon clustering of the ONS-76 tumor spheroid sample (Supplementary Figure S2D), expression of these genes was distributed over all clusters (Supplementary Figure S2E) supporting relative homogeneity of malignant cell states with mostly arbitrary distinctions between clusters, with 2 exceptions. Here, IGFBP2 is linked to neoplasm.