Moreover, EFHD1 overexpression inhibited the cell proliferation index (MCM2 and PCNA) in HCT116 and SW480 cells, whereas EFHD1 knockdown had the opposite effect (Figure 2E), suggesting that EFHD1 has potent antiproliferative effects on CRC cells. Here, EFHD1 is linked to colorectal carcinoma.