Our analysis identified several genes closely associated with tumor cell differentiation: NFKBIA, JUNB, BAMBI, THUMPD3-AS1, SPINK1, EGR1, ATF3, GEM, FOSB, SERPINH1, DNAJA4, MSLN, KRT19, TMSB4X, OCIAD2, SRD5A3, TPPP3, TUBA1A, FOXJ1, and RSPH1. Previous researches have explored the mechanism of action and prognostic value of JUNB30, BAMBI31, ATF332, MSLN33, KRT1934, SPINK135 and EGR136 in NSCLC. This evidence concerns the gene THUMPD3 and non-small cell lung carcinoma.