Our analysis identified several genes closely associated with tumor cell differentiation: NFKBIA, JUNB, BAMBI, THUMPD3-AS1, SPINK1, EGR1, ATF3, GEM, FOSB, SERPINH1, DNAJA4, MSLN, KRT19, TMSB4X, OCIAD2, SRD5A3, TPPP3, TUBA1A, FOXJ1, and RSPH1. Previous researches have explored the mechanism of action and prognostic value of JUNB30, BAMBI31, ATF332, MSLN33, KRT1934, SPINK135 and EGR136 in NSCLC. The gene discussed is EGR1; the disease is neoplasm.