Using AS-MSand PrP aggregation inhibition profiling, we successfully isolatedand identified two bioactive compounds, chlorogenic and neochlorogenicacids, from M. oleifera leaf extracts.These compounds demonstrated the ability to inhibit prion aggregation,prevent autopropagation, and disaggregate PrP fibrils in vitro, highlightingtheir potential as therapeutic agents for prion diseases. This evidence concerns the gene PRNP and prion disease.