To determine if HMGA1 activates the ASCL2 promoter, we cloned the human ASCL2 promoter sequence (–2.5 kb from the TSS) upstream of the luciferase reporter gene and transfected this construct into colon cancer cell lines (SW620, SW480) (Supplemental Figure 11A). The gene discussed is ASCL2; the disease is malignant colon neoplasm.