Silencing HMGA1 in 2 human colon cancer cell lines (SW620, SW480) by lentiviral-mediated delivery of short hairpin RNA (shRNA) or CRISPR/Cas9 represses ASCL2, demonstrating that ASCL2 expression depends on HMGA1 (Figure 12A and Supplemental Figure 9A). The gene discussed is ASCL2; the disease is colonic neoplasm.