Using immunofluorescence staining to target autophagy‐related markers ATG7, ATG16L and Beclin1 in TU686‐Empty Vector and TU686‐CTSL KD cells, confirming the presence of autophagy in laryngeal cancer cells, Knocking down CTSL can reduce the expression of autophagy‐related markers in laryngeal cancer cells. This evidence concerns the gene CTSL and laryngeal carcinoma.