Consistent with the in vivo flow cytometry results (Supplementary Fig. 9), following tumor rechallenge, Allo15CAR33-NKT cells maintained high expression levels of genes encoding effector molecules and cytotoxic molecules (e.g., GZMB, PRF1, KLRC1, and KLRC2), and showed low expression of genes encoding exhaustion markers (e.g., LAG3, CTLA4, and TIGIT) (Supplementary Fig. 10g). Here, PRF1 is linked to neoplasm.