EMILIN1 and neoplasm: The effect of EMILIN-1 on tumor development was evaluated in FaDu cells using the in vivo CAM assay, as illustrated in Figure 8A. The CAM assay was performed by inoculating a three-dimensional culture of transfected FaDu cells into chick embryos on day 7, and the growing tumors were collected on day 14 before preparing formalin-fixed paraffin-embedded tissue blocks.