Specifically, OncoPro medium was supplemented with 10 ng/ml HS FGF-10 for head and neck tumoroid cultures and with 10 ng/ml HS FGF-10 plus 10 nM gastrin I for pancreas tumoroid cultures; colorectal, lung, and breast tumoroids were cultured as described above. This evidence concerns the gene FGF10 and digestive system neoplasm.