Likewise, an unbiased gene expression analysis to identify the top 50 variable genes in our tumor/tumoroid data set followed by hierarchical clustering illustrated a decrease in gene expression levels associated with immune and stromal cells (e.g., CCL3, CCL4, COL1A2, COL1A3) when comparing tumors and derived tumoroids (Supplementary Fig. S3). This evidence concerns the gene COL1A2 and neoplasm.