Piezo1−/− or Piezo1 upregulation by Yoda1 treatment altered SIRT2 and HIF1α expression in neutrophils (Fig. 4C, D), indicating that SIRT2 and HIF1α are likely involved in Piezo1-mediated NET formation in neutrophils during the response to virus infection. The gene discussed is SIRT2; the disease is viral infectious disease.