Although less NET DNA from Piezo1−/− neutrophils than WT control neutrophils induced less expression of TNFα and NOS2 and more expression of IL-10 and CD206 in macrophages, knockdown of cGAS or STING reversed these alterations (Fig. 3G–I), indicating that cGAS-STING is required for M1 macrophage differentiation induced by NET DNA of Piezo1−/− neutrophils during the response to virus infection. This evidence concerns the gene NOS2 and viral infectious disease.