Here, we demonstrate how hypoxic injury and IL-1β/IL-1RI signalling act in combination to induce: (i) PTEC senescence via cell cycle arrest in G2/M phase; and (ii) a potential pro-fibrogenic (TGF-β1/TGFβR1) signalling cascade with capacity to mediate tubulointerstitial fibrosis in human CKD. Here, TGFBR1 is linked to chronic kidney disease.