To evaluate the tumor homing properties of systemically administered CAR T cells, CAR NK cells, and CAR macrophages in an orthotopic, syngeneic solid tumor setting, we characterized the quantity and spatial distribution of intravenously administered fluorescently labeled NKG2D CAR immune effector cells in the brains of GL-261 glioma-bearing mice using ex vivo 3D microscopy (Figure S3A). The gene discussed is KLRK1; the disease is central nervous system cancer.