Simultaneous live-cell imaging with Calcein-AM and lysotracker Red revealed the disappearance of ferroportin in Hepcidin pre-treated MФs fails to pump iron inside LD-R-PV as observed by higher calcein fluorescence similar to LD-S-infection (Figs 4D.ii, upper right panel andS3D, left panel). Here, HAMP is linked to infection.