In order to test the impact of IRS2 on CRC cells, we either overexpressed IRS2 in the IRS2 non-amplified cell lines (denoted HCT116IRS2 HT29IRS2 and SW480IRS2), or silenced it in the IRS2-amplified cell lines (denoted SW403sh-IRS2 SW403sh-IRS2 and LS513sh-IRS2) (Figure 2A and Supplementary Figure S2G) and assessed their oncogenic characteristics. The gene discussed is IRS2; the disease is colorectal carcinoma.