INS and neoplasm: IRS2 protein serves as an adaptor in the insulin and IGF signaling cascades, which are crucial for regulating tumor progression and metabolism.8 We first characterized IRS2 mRNA and protein levels in three IRS2 non-amplified (HCT116, HT29, and SW480) and two IRS2-amplified (SW403 and LS513) CRC cell lines.21 As expected, a correlation between IRS2 mRNA (Supplementary Figure S2E) and protein (Supplementary Figure S2F) expression and IRS2 copy number was observed.