Given that microglia may respond to ATP released by neurons or astrocytes through the P2X7 receptor (P2X7R) in the context of depression,[18, 20] we treated cultured microglia in vitro with the specific P2X7R agonist BzATP to identify which cytokines are upregulated following P2X7R activation. This evidence concerns the gene P2RX7 and major depressive disorder.