Accordingly, the levels of T cell‐mediated cytotoxicity markers such as Perforin and Granzyme B were higher in CD8+ cells co‐cultured with A375P female melanoma cells than in the male counterpart (Fig. 4K,L) and this effect is phenocopied by lactate administration alone or in addition to the A375 female cells co‐culture (Fig. 4M,N). This evidence concerns the gene CD8A and melanoma.