To investigate the existence of a sex‐related difference in the reliance of melanoma cells on glycolysis, we analysed the expression of key glycolytic markers (i.e., HKII, LDH‐A and MCT4) by performing immunohistochemistry (IHC) analysis on a tissue microarray (TMA) containing 80 tissue specimens from male and female melanoma patients (with a similar distribution among age and clinical tumour stage). This evidence concerns the gene SLC16A3 and melanoma.