Specifically, we observed the following changes in RhoA expression of clinical samples: The expression observed in Stage IV, (Sample S2) increased from 0.5 pg mL−1 before stretching to almost 1.55 pg mL−1 after stretching; Stage III (Sample S3) increased from 0.5 to 1.4 pg mL−1; similar phenomena regarding the cancer stage dependency of the RhoA expression was observed in stage II (Sample S1) increased from 0.5 to 0.7 pg mL−1; (Sample S4) increased from 0.5 to 1.0 pg mL−1; and (Sample S5) increased from 0.3 to 0.9 pg mL−1. This evidence concerns the gene RHOA and cancer.