To further investigate the role of peroxisomal β‐oxidation in LA improving As‐induced liver lipid metabolism disorders, this study used TRCDA, an inhibitor of ACOX1, which is a key protein in the peroxisomal fatty acid β‐oxidation pathway, to establish a mechanical model of peroxisomal β‐oxidation. This evidence concerns the gene ACOX1 and Disorder of lipid metabolism.