To clarify the correlation among apCAFs, CD8+ T cells and IFN‐γ+CD4+ T cells in human pancreatic cancer, multiple immune fluorescent staining with MSLN, CD8, CD74 plus PDGFα antibodies and CD4, IFN‐γ, CD74 plus PDGFα antibodies were performed in 30 cases of pancreatic cancer TMA, respectively. This evidence concerns the gene IFNG and pancreatic neoplasm.