To further directly characterize the proportion of potential neoantigen specific CD8+ T cells in the pancreatic cancer, a fluorescently labeled tetramer for immunogenic neoantigen peptide Slc16a13 (p. 234–241: YVHLVANL): H‐2Kb (Slc16a13) was applied to detect the infiltrated CD8+ T cells that expressed Slc16a13‐specific T‐cell receptors (TCRs). This evidence concerns the gene CD8A and familial pancreatic carcinoma.