CD244 and lymphoma: To confirm the localization of 2B4 at a T cell–APC interface, CD8+ OT-I-TCR T cells expressing 2B4-EGFP were conjugated with EL-4 cells, an H-2Kb+ lymphoma cell line with CD48 deleted using CRISPR/Cas9 (EL-4ΔCd48) or reconstituted with CD48 (EL-4ΔCd48-CD48) (Figure S1F).